Search results for " MMPs"

Levels of circulating gelatinases and proteomic correlations in breast cancer patients.

2008

Vanadium perturbs the fertilization outcome and the metalloproteinase activity in sea urchin embryos

2021

Metal toxicology represents a current major topic due to the disper- sion of these elements in the environment. Metals are released from both natural sources and industrial activities. Some of them have also a clinical interest due to their application as metallodrugs (i.e., Pt, Cu, Au, Ru, and Y) or in medical diagnosis (Gd).1,2 Recently, V derivatives are considered as potential therapeutic factors in some diseases (e.g., obesity, diabetes, cancer, neurodegenerative and heart disorders). As a consequence, pharmaceutical residues could repre- sent emerging pollutants of aquatic environments, as wastewater treatment plants do not sufficiently remove these compounds3. Embryonic models repres…

An Alginate/Cyclodextrin Spray Drying Matrix to Improve Shelf Life and Antioxidant Efficiency of a Blood Orange By-Product Extract Rich in Polyphenol…

2017

Alginate and β-cyclodextrin were used to produce easily dosable and spray-dried microsystems of a dried blood orange extract with antidysmetabolic properties, obtained from a by-product fluid extract. The spray-dried applied conditions were able to obtain a concentrate dried extract without the loss of AOA and with TPC and TMA values of 35–40% higher than that of the starting material. They were also effective in producing microparticles with 80–100% of encapsulation efficiency. The 2% sodium alginate was capable of improving the extract shelf life, while the beta-cyclodextrin (1 : 1 molar ratio with dried extract) prolonged the extract antioxidant efficiency by 6 hours. The good inhibition…

WIN modulates osteosarcoma MG63 cell migration by inhibiting MMPs activity and adjusting intra- and extra-cellular SPARC differential expression

2014

Invasion of cancer cells into surrounding tissue is an initial step in tumor metastasis. This event, which requires migration of cancer cells and attachment to extracellular matrix (ECM), is regulated by elements of the local microenvironment, including ECM architecture. After having demonstrated the ability of the synthetic cannabinoid WIN55,512 to induce osteosarcoma MG63 cell death (1), we studied the effects of WIN on MG63 cell migration. Wound healing assay was performed to measure the ability of cells to migrate and fill the gap obtained by physical disruption of cell monolayer (2). We observed a significant delay in wound closure in 5 M WIN treated cells compared to untreated cells …

MMP-2, MMP-9 and MMP-14 in breast cancer: clinicopathological correlations.

2009

New insights into the role of matrix metalloproteinases in heart angiogenesis induced by exercise

2008

Angiogenesis induced by exercise has been observed in both cardiac and skeletal muscle and plays a fundamental role in maintaining tissue function adequate to the increase in metabolic requests. Mechanical and haemodynamic forces are strong starters of angiogenic process via regulation of secondary mediators such as vascular endothelial growth factor (VEGF). A crucial step of vessel sprouting is the degradation of the basement membrane and remodelling of the extracellular matrix (ECM) by matrix metalloproteinases (MMPs). It has long been accepted that MMPs are involved in the angiogenesis, but the exact mechanisms are not well characterized. Cryptic fragments and neo-epitopes released by pr…

Mutant p53 gain of function can be at the root of dedifferentiation of human osteosarcoma MG63 cells into 3AB-OS cancer stem cells

2014

Osteosarcoma is a highly metastatic tumor affecting adolescents, for which there is no second-line chemotherapy. As suggested for most tumors, its capability to overgrow is probably driven by cancer stem cells (CSCs), and finding new targets to kill CSCs may be critical for improving patient survival. TP53 is the most frequently mutated tumor suppressor gene in cancers and mutant p53 protein (mutp53) can acquire gain of function (GOF) strongly contributing to malignancy. Studies thus far have not shown p53-GOF in osteosarcoma. Here, we investigated TP53 gene status/role in 3AB-OS cells-a highly aggressive CSC line previously selected from human osteosarcoma MG63 cells-to evaluate its involv…

Tumor and its microenvironment: a synergistic interplay.

2013

The mutual and interdependent interaction between tumor and its microenvironment is a crucial topic in cancer research. Recently, it was reported that targeting stromal events could improve efficacies of current therapeutics and prevent metastatic spreading. Tumor microenvironment is a "complex network" of different cell types, soluble factors, signaling molecules and extracellular matrix components, which orchestrate the fate of tumor progression. As by definition, cancer stem cells (CSCs) are proposed to be the unique cell type able to maintain tumor mass and survive outside the primary tumor at metastatic sites. Being exposed to environmental stressors, including reactive oxygen species …

Manganese overload affects p38 MAPK phosphorylation and metalloproteinase activity during sea urchin embryonic development.

2014

Abstract In the marine environment, manganese represents a potential emerging contaminant, resulting from an increased production of manganese-containing compounds. In earlier reports we found that the exposure of Paracentrotus lividus sea urchin embryos to manganese produced phenotypes with no skeleton. In addition, manganese interfered with calcium uptake, perturbed extracellular signal-regulated kinase (ERK) signaling, affected the expression of skeletogenic genes, and caused an increase of the hsc70 and hsc60 protein levels. Here, we extended our studies focusing on the temporal activation of the p38 mitogen-activated protein kinase (p38 MAPK) and the proteolytic activity of metalloprot…

Gli aneurismi dell'aorta addominale

2009

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